ABSTRACT
Objective:To investigate the changes and clinical significance of serum S100β and neuron-specific enolase (NSE) levels of children with acute brain injury(ABI).Methods:100 children with ABI treated in the pediatric intensive care unit (PICU) of the Affiliated Hospital of Inner Mongolia Medical University from June 2019 to June 2020 were prospectively selected as the ABI group, and 30 normal children in the children′s health clinic of the hospital were selected as the control group. The serum S100β and NSE levels of all subjects was detected. According to the Glasgow Coma Scale (GCS), children with ABI were divided into severe brain injury group ( n=26), moderate brain injury group ( n=35) and mild brain injury group ( n=39). The prognosis of children with ABI after 3 months of treatment was evaluated according to the Glasgow prognosis scale (GOS) and they were divided into poor prognosis group ( n=26) and good prognosis group ( n=74). The relationship between serum S100β and NSE levels and the severity and prognosis of children with ABI was analyzed. Results:The serum S100β and NSE levels in the ABI group were significantly higher than those in the control group, and the serum S100β and NSE levels in children with ABI increased with the severity of injury and poor prognosis ( P<0.05). Pearson correlation analysis showed that serum S100β and NSE levels in children with ABI were positively correlated with GCS scores ( r=0.521, 0.643, P<0.05). Multiple logistic regression analysis showed that glucose(GLU) ( OR=1.631, 95% CI: 1.278-2.082), S100β ( OR=1.907, 95% CI: 1.558-5.877), NSE ( OR=2.896, 95% CI: 1.193-7.029) were independent prognostic factor in children with ABI ( P<0.05). Receiver operating characteristic (ROC) curve showed that the sensitivity, specificity and accuracy of serum S100β+ NSE [area under curve (AUC)=0.932, 95% CI: 0.875-0.969] in predicting the poor prognosis of children with ABI were higher than those of serum S100β(AUC=0.728, 95% CI: 0.643-0.803), NSE (AUC=0.808, 95% CI: 0.729-0.871) alone. Conclusions:The levels of serum S100β and NSE in children with ABI aresignificantly increased, which are closely related to the severity of the disease and prognosis. They can be used as predictors of poor prognosis in children with ABI. Combined detection can enhance the diagnostic value.
ABSTRACT
The ultrastructure of hepatic fat-storing cells in rat embryos was investigatedwith transmission electron microscope.It is considered that the rat fat-storingcells originate from mesenchymal cells.As the embryo develops,the fat-storing cell decreases in size gradully,this isespecially obvious at the time of birth.This may be related with the function of thistype of cell.In the hepatic fat-storing cell in embryo of 10 to 12 days,the roughendoplasmic reticulum has developed,the cell is rich in free ribosomes,and collagenfibrils may be observed in Disse's spaces.This indicates that the fat-storing cellshave possessed the function of synthesizing collagen.In embryo of 13 to 15 days,fat droplets appear in a few fat-storing cells and smooth endoplasmic reticulum, glycogen granules and pinocytosis may be seen at this time.It is suggested that thefunction of the uptake and storage of vitamin A may appear at that time too.There are desmosomes between the fat-storing cell and the hepatocyte.On the 16to 18 days,desmosomes are also present between the fat-storing cell and endothelialcell.This indicates that fat-storing cells are not free cells,but are bound to hepa-tocytes and endothelial cells via cellular junctions,At that time,microfilaments andmicrotubules are found in fat-storing cells,they are the skeleton of the cells andthey may be related to the regulation of the size of the sinusoids.In thedevelopment of embryo,the number of fat-storing cells and fat droplets have notincreased markedly,therefore,it is thought that they are increased after birth.